Long-read sequencing technologies, also known as the third-generation sequencing, overcome some the limitations of the second-generation sequencing technologies in some situations, while maintaining the advantages of the NGS, such as high throughput, cost-efficient and accuracy. With the ability to produce much longer reads, the instruments of Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) decrease the needs for massive computational requirements of genome assembly, transcriptome analysis, and metagenomics. Also, the ability to detect epigenetic markers directly, provide competitive alternative for epigenomic studies.

APPLICATIONS

• De-novo sequencing of large genomes
• Population-scale sequencing
• Structural variation analysis
• Haplotype phasing
• Full length transcriptome sequencing (IsoSeq)
• DNA modifications
• Simultaneous Epigenetic Characterization
• Highly multiplexed small genomes or targeted regions
• Whole transcriptomes (direct RNA or cDNA)

Specifications: Pacific Biosciences Sequel IIe

Specifications: PromethION